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KMID : 0605219880050010007
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Pusan Dental Journal
1988 Volume.5 No. 1 p.7 ~ p.11
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Effect of Thiopental Sodium on the Fluidity of Synaptosomal Plasma Membrane Vesicles Isolated from Bovine Brain
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Abstract
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Theories for the mechanism of action of barbiturates include the specific receptor theory, the membrane expansion theory, the protein perturbation theory, and the annular transition model. Neither of the above proposals can satisfactorily explain the pharmacological effects of these drugs and most of them are based on the experimental results obtained with model membranes. To get a better insight into the molecular mechanism of action of these drugs, we isolated synaptosomal plasma membrane vesicles (SPMV) from fresh bovine cerebral cortex and examined the effect of thiopental sodium on the fluidity of SPMV. Intramolecular excimer formation with 1, 3-di (1-pyrenyl) propane (Py-3-Pyl and fluorescence polarization with 1, 6-diphenyl-1, 3, 5-hexatriene (DPH) have been used for fluidity measurements. Thiopental sodium increased the excimer to monomer fluorescence intensity ratio (I´/I) of Py-3-Py and decreased the fluorescence polarization of DPH at 371C These results indicate that thiopental sodium fluidized the hydrophobic region of SPMV and might increase the rotational rate of acyl chain of the lipid bilayer.
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KEYWORD
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